Identification of Enterococcus faecalis antigens specifically expressed in vivo
이석우, Shet Uttom K., 임현필, 윤귀덕, 강성수, 김세은,
소속 상세정보
이석우 ( Lee Seok-Woo ) - Chonnam National University School of Dentistry Department of Dental Education
( Shet Uttom K. ) - Chonnam National University School of Dentistry Department of Oral and Maxillofacial Surgery
임현필 ( Lim Hyun-Pil ) - Chonnam National University School of Dentistry Department of Prosthodontics
윤귀덕 ( Yun Kwi-Dug ) - Chonnam National University School of Dentistry Department of Prosthodontics
강성수 ( Kang Seong-Soo ) - Chonnam National University College of Veterinary Medicine
김세은 ( Kim Se-Eun ) - Chonnam National University College of Veterinary Medicine
KMID : 1034420150400040306
Abstract
Objectives: Molecular mechanism of the pathogenicity of Enterococcus faecalis (E. faecalis), a suspected endodontic pathogen, has not yet been adequately elucidated due to limited information on its virulence factors. Here we report the identification of in vivo expressed antigens of E. faecalis by using a novel immunoscreening technique called change-mediated antigen technology (CMAT) and an experimental animal model of endodontic infection.
Materials and Methods: Among 4,500 E. coli recombinant clones screened, 19 positive clones reacted reproducibly with hyperimmune sera obtained from rabbits immunized with E. faecalis cells isolated from an experimental endodontic infection. DNA sequences from 16 of these in vivo-induced (IVI) genes were determined.
Results: Identified protein antigens of E. faecalis included enzymes involved in housekeeping functions, copper resistance protein, putative outer membrane proteins, and proteins of unknown function.
Conclusions: In vivo expressed antigens of E. faecalis could be identified by using a novel immune-screening technique CMAT and an experimental animal model of endodontic infection. Detailed analysis of these IVI genes will lead to a better understanding of the molecular mechanisms involved in the endodontic infection of E. faecalis.
키워드
Antigen; Enterococcus faecalis; In vivo; Pulpitis; Virulence factor
원문 및 링크아웃 정보
등재저널 정보